Research Article

Antimicrobial and Phytochemical Screening of Combretum Leaf Against Two Pathogens

Abstract

The combretum leaf extracts were investigated for phytochemical screening and antimicrobial activity on Staphylococcus aureus and Eseherichia coli by agar well diffusion method using Augmentin as standard antibiotics. The phytochemical screening revealed the presence of saponins, flavanoids, alkaloids, terpenoids, tannins whereas glycosides and steroids are absent in ethanol, methanol and aqueous extracts respectively. The ethanol, methanol and aqueous extracts were used in varying concentrations. The zone of inhibition was observed in both strains and the three extracts were found to show a very high antimicrobial activity but the ethanol extracts possessed more activity than the methanol and aqueous extracts, thus they were found to have Inhibitory action. The minimum inhibitory concentration (MIC) was found to range from 0.45 to 2.5mg/ml respectively. combretum leaf extract is thus recommended for the treatment of human infections caused by these organisms.

Authors affiliation:

Ike Ozoemena Christian (ORCID): Department of Industrial Chemistry, Enugu State University of Science and Technology, Enugu State, Nigeria.
Ogbuanu Cyril Chinedu (ORCID): Department of Industrial Chemistry, Enugu State University of Science and Technology, Enugu State, Nigeria.
Okoye Nebeolisa Johnson (ORCID): Department of Industrial Chemistry, Enugu State University of Science and Technology, Enugu State, Nigeria

How to Cite: I.O. Christian, O.C. Chinedu and O.N. Johnson. Antimicrobial and Phytochemical Screening of Combretum Leaf Against Two Pathogens. Journal of Applied Health Sciences and Medicine, 6(6):24–28, 2026. https://doi.org/10.58614/jahsm665