Research Article
Genetic investigation of virulence factors genes among Pseudomonas aeruginosa isolated from clinical sources
- By Mohammedbaqeri Hayder Alkam Thebhawe, Nabil Salim Saaid Tuwaij - 12 Jun 2025
- Journal of Applied Health Sciences and Medicine, Volume: 5, Issue: 6, Pages: 8 - 12
- https://doi.org/10.58614/jahsm562
- Received: 06.03.2025; Accepted: 21.05.2025; Published: 12.06.2025
Abstract
Pseudomonas aeruginosa is considered one of the most common causes of infection, especially in immunocompromised patients. Therefore, this study aimed to detect the virulence factor genes in Pseudomonas aeruginosa isolates. The isolates were collected from different sources: 18/100 (18%) burn patients, 13/100 (13%) diabetic foot ulcer patients, and 2/100 (2%) patients suffering from urinary tract infections. The growth rate of Gram-positive bacteria was 79 (26.33%), while the rate of Gram-negative bacteria was 168 (56%). Results of antibiotic susceptibility showed that the highest bacterial resistance was Cefepime 33(100%) while the least resistance was Meropenem reached 16(48.48%). Results of Polymerase chain reaction (PCR) showed that 29 (87.87%), 9 (27.27%), 29(87.87%), 33(100%), 33(100%) and 17(51.51%) of P. aeruginosa isolates had pilB, pilA, lasA, lasB, algD, and nan-1 genes, respectively in this work.
Author's Information:
Mohammedbaqeri Hayder Alkam Thebhawe. Department of Biology Faculty of Science, University of Kufa, Iraq.
Nabil Salim Saaid Tuwaij. Department of Biology Faculty of Science, University of Kufa, Iraq.
How To Cite: M.B.H. Ahebhawe, N.S.S. Tuwaij. Genetic investigation of virulence factors genes among Pseudomonas aeruginosa isolated from clinical sources. Journal of Applied Health Sciences and Medicine, 5(6):8–12, 2025. https://doi.org/10.58614/jahsm562